Coding

Part:BBa_K4998025:Design

Designed by: Rafail Andreou   Group: iGEM23_thessaloniki   (2023-10-09)


VapC15 gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 66
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cyanobacteria have endogenous toxin-antitoxin systems. Taking that into consideration we tried to find a system that did not already exist in our strain of cyanobacterium.


Source

Synechocystis sp. PCC 6803

References

[1] Fei, Q., Gao, E.-B., Liu, B., Wei, Y., & Ning, D. (2018). A toxin-antitoxin system Vapbc15 from synechocystis sp.. PCC 6803 shows distinct regulatory features. Genes, 9(4), 173. https://doi.org/10.3390/genes9040173